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SMO(A68V)

Anti-SMO(A68V) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26425

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   SMO(A68V) 小鼠单抗
货号:   26425
规格:   100 µL
基因符号:   SMO; MRD21
描述:   SMO(A68V) 小鼠单抗
背景:   Smoothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
免疫原:   A synthetic peptide from the internal region of SMO which includes the mutation of A68V, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes A68V mutant, but not wild type SMO of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant SMO(A68V) and wild type proteins. Purified His-tagged SMO(A68V) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-SMO(A68V) monoclonal antibody (货号:26425).
Immunofluorescence:  Immunofluorescence of cells expressing SMO proteins with Anti-SMO(A68V) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMO (WT) plasmid (left column) or pCDNA3-GFP-SMO(A68V) plasmid (right column), then fixed and stained with Anti-SMO(A68V) monoclonal antibody (货号:26425).
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